Thursday, August 27, 2020
Aristotleââ¬â¢s Politics - The Good Man Should Not Rule the City Essay
Aristotleââ¬â¢s Politics - The Good Man Should Not Rule the City Aristotle battles that the great man is not at all like the productive member of society in manners he goes an incredible length to outline. He recognizes the two to encourage his later contentions concerning the proper allotment of power to the legitimate ruler, who he accordingly asserts is the acceptable man who exceeds expectations all others in every single perspective. Aristotle's differentiation further prompts the idea that he advocates a monarchial type of constitution, for the standard of a solitary decent man is equal to a constitution of authority. This can be determined through the accompanying thinking. Aristotle is persuaded that the productive member of society can so be characterized distinctly comparable to the constitution he is a component of: 'The greatness of the resident must be a greatness comparative with the constitution (1276b16).' The great man then again, 'is a man supposed in ethicalness of a solitary supreme greatness (1276b16).' He further affirms that the productive member of society 'must have the information and limit essential for administering just as for being ruledãâ"a decent man will likewise require both (1277b7~1277b16).' From these finishes of Aristotle, it is apparent that the great man and the productive member of society contrast in the way of their greatness, however not in their ability for administering or being dominated. It ought to in this manner follow that there ought not exist hindrances to the decision by the productive member of society in the city rather than the decision by the great man because of the way that they are indistinguishable in their skill to run the show. Be that as it may, Aristotle in his later contentions, crowns the great man as ruler: 'in the best constitutionãâ"there is somebody of remarkable greatness. What could possibly be done that case? No one wou... ...scussed). The support of the great man in turning into the preeminent teacher can be made in the accompanying manner. Since all completely great men (great men) show up at their greatness through the procedure of instruction, that is, they are not intrinsically amazing, their endeavors ought to be coordinated toward the copying of their greatness in the offspring of the city, for they are the ones who know best the way toward getting magnificent. As such of training, the youngsters (being future residents) will grow up to turn out to be acceptable men and productive members of society, and in this way the future city will include numerous likely rulers. The great man through instruction, will contribute towards the decision of the city in a roundabout way in such a case, and not straightforwardly as Aristotle claims he ought to do. Works Cited Aristotle. Poetics. Trans. Gerald F. Else. Ann Arbor: Ann Arbor Paperbacks, 1990.
Saturday, August 22, 2020
Old Man And The Sea :: essays research papers
Throughout everyday life, one will experience various stages throughout everyday life. Early stages, Youth , Adulthood, and Mature Age are on the whole key stages. As one develops, they develop through these different stages. At the point when one arrives at mature age, there is regularly a ton of uncertainty encompassing their lives. Tranquility, and autonomy are regularly the two most addressed. These are a few inquiries that Santiago needs to pose to himself as well. Ã Ã Ã Ã Ã In the novel The Old Man And The Sea, Ernest Hemingway builds up the idea of man going to the acknowledgment that as he ages, his reliance on others will increment. The utilization of representation is key in indicating how this is undoubtedly obvious. The battle with the Tiburon speaks to the mental battle that Santiago is having with himself. The Tiburon is likewise utilized as an illustration for Santiago’s life. The kid in the story matches what Santiago’s life used to be. Ã Ã Ã Ã Ã The battle with the Tiburon speaks to the battle that Santiago is having with himself. The consistent battle causes Santiago to understand that he is no longer as youthful as he might suspect he is and he should depend on the assistance of others. This is indicated when Santiago is fighting the Tiburon. “ ‘Bad news for you fish’, he said and moved the line over the sacks that secured his shoulders. He was agreeable, however languishing, in spite of the fact that he didn't admit to the enduring by any stretch of the imagination. ‘ I am most certainly not religious...but I will say Ten Hail Marys that I should get this fish’... ‘Hail Mary brimming with Grace the Lord is with thee. Favored craftsmanship thou among ladies and favored is the product of thy belly, Jesus. Blessed Mary, Mother of God petition God for us heathens now and at the hour of death, Amen.’ Then he included. ‘ Blessed Virgin, appeal to God for the passing of this fish, superb as he is.’ '; [ Hemingway 64-65] Ã Ã Ã Ã Ã This quote shows that the elderly person is compelled to disrupt the guidelines of mental soundness and converses with himself just as the fish which can't hear him. The elderly person contemplates internally that the fish is a “ “ God dreading '; fish and by saying the Hail Mary, the fish will yield and let himself be gotten. In the wake of saying the Hail Mary the Old Man attempts to persuade himself that his recently harmed hand is OK, when truly it isn't. Ã Ã Ã Ã Ã In another piece of the story Santiago concedes that he is losing his mental soundness. “He didn't need to take a gander at the fish. He realized that half of him had been annihilated '; [Hemingway 114].
Friday, August 21, 2020
Facebook Vs Google Plus [INFOGRAPHIC]
Facebook Vs Google Plus [INFOGRAPHIC] Make Money Online Queries? Struggling To Get Traffic To Your Blog? Sign Up On (HBB) Forum Now!Facebook Vs Google Plus [INFOGRAPHIC]Updated On 16/01/2018Author : Aman Arora @ Tech HowTo's BlogTopic : InfographicShort URL : https://hbb.me/2DAVbGZ CONNECT WITH HBB ON SOCIAL MEDIA Follow @HellBoundBlogSince the launch of Google Plus, internet critics started comparing Facebook and Google Plus. If you dont know what it is then let me tell you, it is a brand new social network created by Google. Everybody wanted to compare the features of these 2 giants.Therefore guys at The Tech Addicts made a graphic to compare various features of these social networks. They both have advantages and disadvantages, and it is up to you to decide where you want to stay longer.Check out the interesting infographic below to see the analysis between the two social networking sites.Infographic Credit : The Tech AddictsREADEarth Day: Internet Killing The Planet? [INFOGRAPHIC]
Monday, May 25, 2020
Let Me Make A Declaration Of Modesty - 874 Words
Before I begin, let me make a declaration of modesty. I am, by no account, a legal expert, and, at this point, having a career as illustrious and fruitful as President Garrettââ¬â¢s is a mere dream. She is, of course, far more intelligent, informed, and experienced than I in matters relating to law and, in particular, the Supreme Court of the United States, and it is for this reason that I write this article not in criticism, but in genuine confusion. During the Question Answer portion of the September 18th faculty panel discussion regarding ââ¬Å"Democracy Inequality,â⬠President Garrett and Professor Robert H. Frank, management and economics, criticized the Supreme Courtââ¬â¢s holding in Citizens United v. Federal Election Commission (2010) that the First Amendment permits independent corporate political expenditures. President Garrett and Professor Frank chastised the ruling for its pernicious consequences on American democracy. Professor Frank derisively highlighted the rulingââ¬â¢s establishment of corporate personhood, and President Garrett pondered how the five-justice majority (Roberts; Scalia; Kennedy, opinion author; Thomas; Alito) could possibly have concluded that unrestricted political donations would not inherently precipitate bribery, for which there exists, in the Presidentââ¬â¢s words, a ââ¬Å"compelling State interestâ⬠to prevent. Ultimately, the general panel consensus was twofold: Citizens United was wrongly decided, and the case should be overturned. First, ProfessorShow MoreRelatedIn Jane Austenââ¬â¢s Persuasion love and family is a major thing I learned to see and control just1300 Words à |à 6 Pagesnever forgave Anne for breaking his heart. He flirted with other women in front of Anne to make her jealous, typical man right? Is it mans nature to forget the woman he loves sooner than woman forgets man? A quote that stood out to me is this. ââ¬Å"The one claim I shall make for my own sex is that we love longest, when all hope is gone.â⬠The reason this stood out to me is because I believe this is very true. Men let love go while us females hold on. Love fills us up, itââ¬â¢s like we cannot live without itRead MoreThe Abu Bakr Masjid : The Islamic Center Of San Diego1895 Words à |à 8 Pageswaiting for me outside of the mosque. The first matter he explained was that it was considered proper to enter the masjid (mosque) with my right foot first and exit with my left foot first. Furthermore, I was also explained that in order to enter the prayer hall, I needed to be in a state of ritual purity. This purification consists of ablutions, called wuduââ¬â¢, which involve the washing of the feet, hands, head and face. Once I completed the ablutions, Imam Taha Hassane instructed me to take offRead More The Themes in Oedipus Rex Essay2792 Words à |à 12 Pagescontains one main theme, which this essay will conside r. The theme is the general doctrine or belief implicit in the drama, which the author seeks to make persuasive to the reader (Abrams 170). à In ââ¬Å"Sophoclesââ¬â¢ Moral Themesâ⬠Robert D. Murray Jr. cites a critic who is strictly moralist in the interpretation of the theme of Oedipus Rex: à Let C. M. Bowra speak for the moralists: à The central idea of a Sophoclean tragedy is that through suffering a man learns to be modest beforeRead MoreEssay on Tragic Flaws in Oedipus the King3561 Words à |à 15 Pagesthen, Oedipusââ¬â¢ misfortune was directly ocasioned by some serious hamartia. . . . The word hamartia is ambiguous: in ordinary usage it is sometimes applied to false moral judgments, sometimes to purely intellectual error - the average Greek did not make our sharp distinction between the two(18-19). This view of a tragic flaw, moral or intellectual in nature, within the protagonist is not shared by all literary critics. Robert D. Murray, Jr. in ââ¬Å"Thought and Structure in Sophoclean Tragedyâ⬠givesRead More Mythology in Oedipus Rex Essays3980 Words à |à 16 Pagesproblem, which is admirably presented in this individual shape(31). à The Homeric myth provides the story of the fall of a man from prosperity to adversity. Sophocles takes the myth and dramatizes it in such a way that every word and action makes an impact on the audience. The audience can see the error that beset Oedipus, and see his living state of mind as reflected in themselves; they empathize with him in his moral decline. à Aristotleââ¬â¢s analysis admits that the tragic flaw in OedipusRead More Mythology in Oedipus Rex Essay examples4094 Words à |à 17 Pagesproblem, which is admirably presented in this individual shape(31). à The Homeric myth provides the story of the fall of a man from prosperity to adversity. Sophocles takes the myth and dramatizes it in such a way that every word and action makes an impact on the audience. The audience can see the error that beset Oedipus, and see his living state of mind as reflected in themselves; they empathize with him in his moral decline. à Aristotleââ¬â¢s analysis admits that the tragic flaw in OedipusRead More Destiny, Fate, Free Will and Free Choice in Oedipus the King3894 Words à |à 16 PagesDelphi, addresses a brief prayer to King Apollo as the ultimate source of assistance in time of trial: ââ¬Å"O King Apollo! may his joyous looks /Be presage of the joyous news he brings!â⬠Creon brings to Thebes the message of the gods from the oracle: ââ¬Å"Let me report then all the god declared. /King Phoebus bids us straitly extirpate /A fell pollution that infests the land, /And no more harbor an inveterate sore.â⬠à The gods know that Oedipus is a ââ¬Å"pollution,â⬠a ââ¬Å"sore,â⬠which must be gotten ridRead More Dramatic Irony in Oedipus Rex Essay3192 Words à |à 13 Pagesto be grossly inappropriate to the actual circumstances, or expects the opposite of what we know that fate holds in store, or says something that anticipates the actual outcome, but not aat all in the way that the character intendsâ⬠(137). à Let us explore that feature in Sophoclesââ¬â¢ Oedipus Rex. à à From the very outset of the tragedy, Sophocles relies on heavy irony. E. T. Owens in ââ¬Å"Drama in Sophoclesââ¬â¢ Oedipus Tyrannusâ⬠comments: à We must bear in mind that Sophocles buildsRead MoreA Great Translator s Reflections On Oedipus The King1999 Words à |à 8 PagesReflections on Oedipus the King,â⬠Gilbert Murray, professor at Oxford University in England, cites structure as one of the reasons why he chose Oedipus Rex as a work of translation: On the whole, I can only say that the work of translation has made me feel even more strongly than before the extraordinary grip and reality of the dialogue, the deftness of the construction, and . . . the unbroken crescendo of tragedy from the opening to the close (105). . . . But Sophocles worked by blurring his structuralRead More Comparing A Streetcar Named Desire and Cat on a Hot Tin Roof1765 Words à |à 8 Pagesbrute force. Sometimes refusing to make choices is powerful. Forcing other to make decisions and thus never shouldering the responsibility is a form of dominance. Brick proudly proclaims Maggie laid down the law to me - said now or never, and so I married M aggie (Williams, Cat 37). She has to accept a non-sexual marriage or she has to be the one decide to leave. In the end Maggie, wanting to sire an heir and secure her position in the family, forces Brick to make love to her. He doesnt fight her
Thursday, May 14, 2020
The Spanish Conquest - 1711 Words
Although some may consider the fall of the Nahua and Inca empires an encounter of the Spanish, the meeting of the two cultures was a conquest because the Spanish brutally defeated and took over the indigenous cultures with the help of many advantages. There are three major factors which contributed to the successful conquest between the Spanish and the Nahuas, also known as the Aztecs, and Incas. First, the Spanish leaders had experience in forming alliances with indigenous people. Second, the Spanish had superior weaponry and military advantage, like steel, horses, and guns which made their weapons stronger and much more deadly. Third, the Spanish were physically capable of interacting with the indigenous people without suffering fromâ⬠¦show more contentâ⬠¦If granted, Pizarro would set Atahualpa free. After giving Pizarro the amount of gold he wanted, expecting to be freed, Atahualpa was strangled. From the Nahua perspective, they were clearly conquered because Tenochtitla n had been one of the most powerful cities at the time. Even though the boys were educated in battle at a young age and outnumbered the Spanish completely, they could not compare to the Spanishââ¬â¢s experience and advanced weaponry. Although the Spanish had many advantages and factors that aided their success, the Nahuaââ¬â¢s impressive civilization was just too vulnerable due to their own emperor, Moctezuma, inviting the enemy inside their home; the Nahua were the weaker opponent. They felt betrayed by Cortà ©s, since they thought he was the long expected Quetzalcoatl. A feeling of betrayal especially arose after Cortà ©s conquered the Nahua after they had showered him in gold, lavish gifts and openly invited him and his men into the city. The Incas too, felt betrayed. After the Peruvian conquest, which started in Panama and worked its way down to Peru, the Incas were also brutally defeated due to their disadvantage of a broken civilization because of the civil war and bec ause of the infectious diseases that depleted the Incan population. Again, although the Spanish had many advantages that helped them succeed, the Incas were the weaker opponent, like the Nahua. The Spanish, of course, viewed years 1519 through 1521 as a conquest. With mostlyShow MoreRelatedEffects Of Spanish Conquest1298 Words à |à 6 Pagesa twenty million prior to Spanish conquest (Marr and Kiracoffe 2000). These civilizations were also onlookers to one of the worst demographic tragedies in history seeing population losses of almost ninety percent, down to one million occupants a century after conquest (Marr and Kiracoffe 2000). These demographic tragedies were in the form of epidemics of both New and Old World origin and as a result of and major contributing factors to the success of the Spanish Conquest of the region. As the SpaniardsRead MoreHistory: Spanish Colonization of the Americas and Spanish Conquest1021 Words à |à 5 PagesThe Conquest and Colonization of the Caribb ean and Yucatan by European, Resulted in significant cultural, biological environmental changes to both regions Maggie Jim Professor Ancient History [Date] 1. Briefly discuss (but in detail) the similarities/differences between the initial Spanish colonization of the Caribbean and the Yucatan. In your discussion include the initial reaction of the Taino and Maya to the presence of the Spanish and the rationalRead MoreHistory: Spanish Colonization of the Americas and Spanish Conquest1021 Words à |à 5 PagesThe Conquest and Colonization of the Caribbean and Yucatan by European, Resulted in significant cultural, biological environmental changes to both regions Maggie Jim Professor Ancient History [Date] 1. Briefly discuss (but in detail) the similarities/differences between the initial Spanish colonization of the Caribbean and the Yucatan. In your discussion include the initial reaction of the Taino and Maya to the presence of the Spanish and the rationalRead MoreTaking a Look at the Spanish Conquest1313 Words à |à 6 PagesIntroduction: THE SPANISH CONQUEST of the Americas is an interesting story of exploration, wealth, greed, devastation and death. The Aztec civilization, which lived in what we know today as central and South America, began to come under threat from European explorers during the late 15th century. The Aztec civilization was one of the most spectacular in the world, and at its heart was the masterpiece of the Aztec capital, Tenochtitlan. However, the prosperity and wonder of the Aztecs came to an endRead MoreThe Spanish Religious Conquest Of America1739 Words à |à 7 Pagessociety today. The Spanish religious conquest was one of the first early history examples that played a big role in America. As soon as the Spanish landed in New Spain it changed the Native Mexican culture. The consequence of the spiritual conquest was the loss of the native people beliefs, buildings, and customs. However, the Native Mexicans did not respond well to the conquest, it caused them to rebelling against the Spanish, but with time they started to accept the Spanish ways. WhenRead MoreImpact of the Spanish Conquest on the Aztecs. Essay example1418 Words à |à 6 Pagespracticed an incredibly wealthy lifestyle. Nonetheless, this rule began to deteriorate when Spanish explorers disembarked at Tabasco and Vera Cruz on April 21st 1519. When the Spanish voyagers first arrived, they were welcomed warmly, respectfully and received Godlike treatment. Montezuma, the ruler at that time, believed that the Spanish military leader, Hernà ¡n Cortà ©s, was the great god Quetzalcoatl. The Spanish took advantage of this Aztec belief and conquered Mexico within two years. By 1521, theRead MoreDiego Valesquez followed Columbus in the Spanish Conquest935 Words à |à 4 PagesAfter Columbus arrived, Spanish conquest followed under the guise of Diego Valesquez, a move that brought on the eradication of the native Cuban population as a result of European diseases. The 1800ââ¬â¢s brought on a large sugarcane industry in Cuba, which fueled the Africa n slave trade in the country. After Columbus discovered the New World, Spain immediately sent Diego Velazquez de Cuellar (not to be confused with Diego Velazquez the painter) to establish a Colony on Cuba, Baracoa(1511). A rapidRead MoreAnalysis Of Bernal Diazs Seven Myths Of The Spanish Conquest829 Words à |à 4 Pages The Florentine Codex classily illustrated numerous battles and confrontations between the Mexicas, the Spanish, and other native peoples. The theme of miscommunication presented by Hernan Cortes is supported by Bernal Diazââ¬â¢s account in Seven Myths of the Spanish Conquest. It is worth mentioning the fact that Bernal Diaz describes Spaniards going into Mexico under Hernan Cortes. The narrator describes these events as an eyewitness, who saw what happened and attempted to reveal the truth to othersRead MorePeru s Indian Peoples And The Challenge Of Spanish Conquest1422 Words à |à 6 Pagestext, Peruââ¬â¢s Indian Peoples and the Challenge of Spanish Conquest, highlights from beginning to end Spainââ¬â¢s conquest of the Andean people while articulating the transformation of the relationship between these two peoples. He illustrates how Spainââ¬â¢s efforts toward colonialism of the Andes, transformed the natives from a self-sufficient group of people into an oppressed ca ste system known as ââ¬Å"Indians.â⬠Additionally, he introduces the idea that the Spanish needed to break the indigenous and gain theirRead MoreCortes Conquers the Aztecs: Spanish Conquest or Indian Civil War781 Words à |à 4 PagesKristen Santoyo Chicano Studies 101 Tuesday/Thursday 9 AM Mid-Term Essay Question The Spanish conquests of Montezuma and the Aztecs were both a Spanish conquest as well as an Indian civil war. The thing that ties them both together is the Conquistador Hernan Cortes. You could argue for both sides as to which played a more significant part in the fall of the Aztecs, however, I feel they both played an equal role in the defeat of the Aztecs and the fall of Tenochititlan to the Spaniards. When
Wednesday, May 6, 2020
Harlem And The Harlem Renaissance - 1430 Words
The Great Migrations was the movement or relocation of over 6 million blacks from Southern rural regions to Northern urban areas. Looking to leave behind the racial tension of the south, and with hopes of new job opportunities in the north, many blacks moved in to urban cities such as Chicago, Detroit, and Harlem. Out of these northern metropolises, the most popular was Harlem; ââ¬Å"here in Manhattan (Harlem) is not merely the largest Negro community in the world, but the first concentration in history of so many diverse element of Negro lifeâ⬠(1050). Harlem became the mecca of black people, and between the years of 1920 and the late 1930s it was known as the Harlem Renaissance. The Harlem Renaissance, brought artiest, poets, writers,â⬠¦show more contentâ⬠¦Despite their many similarities, they have an equal amount of differences, which add to their experience in the black community. Arna Bontemps was born on October 13, 1902 in Alexandria, Louisiana. To two loving par tners, his father descendent of slaves and his mother was English and Cherokee Indian bloodline. When Bontemps was a young boy, around the age of three his family moved to Los Angles California. Growing up he was considered middle class and his partners influenced him greatly as they were involved in the arts. Arna went on to attend Pacific Union College now known as UCLA and obtained a degree win teaching where he went on to become a teacher in Harlem in 1924. After his death, his Harlem home was into an African American museum. In looking at Langston Hughes, we see that we were born on February 1, 1902 in Joplin, Missouri. Like Arna, he was not fully black as both of his partners were biracial. However, unlike Bontemps, his partners separated when we was young, and was mainly raised by his grandmother in Cleveland, Ohio. Hughes also obtained a higher education at Lincoln University and Columbia University. Having a love to travel, he spent much time on the road until he made is h ome in Harlem. From looking at the background of these men, we see that they have many similarities, however we also see big differences in their upbringing which all played a role in how they experienced being black, but moreShow MoreRelatedHarlem And The Harlem Renaissance Essay2269 Words à |à 10 Pagessouthern African Americans migrated to a city called Harlem in New York. They relocated due to dogmatism and intolerance of melanin diverging out the of pores of many white southerners. The African Americans who migrated found new opportunities both economic and artistic that resulted to the creation of a stable middle class Black ââ¬âAmericans (Dover, 2006). This was the Harlem Renaissance a cultural, social, and artistic explosion. The core of Harlem expressed by Alain Locke is that through art, ââ¬Å"negroRead MoreThe Harlem Renaissance850 Words à |à 4 Pages Giselle Villanueva History IB Mr. Flores February 7, 2016 Period 4 Word Count: 693 Harlem Renaissance The Harlem Renaissance was the first period in the history of the United States in which a group of black poets, authors, and essayist seized the opportunity to express themselves. The Great Migration was the movement of six million African Americans from the rural South to the cities of the North during 1916 to 1970. Driven from their homes by unsatisfactory economic opportunities and harsh segregationistRead MoreThe Harlem Renaissance1154 Words à |à 5 PagesIV AP 16 November 2015 The Harlem Renaissance The early 1900s was a time marked with tragedy in America. Started and ended with the Great Depression in between, it was not America s finest moment. Prohibition was in place, the Klu Klux Klan was still marching, and the Lost Generation was leaving for Paris. But despite the troubling times, people still found beauty and meaning in the world around them. They still created art and celebrated life. The Harlem Renaissance was an artistic and literaryRead MoreThe Harlem Renaissance1209 Words à |à 5 Pagesï » ¿The Harlem Renaissance represents the rebirth and flowering of African-American culture. Although the Harlem Renaissance was concentrated in the Harlem district of New York City, its legacy reverberated throughout the United States and even abroad, to regions with large numbers of former slaves or blacks needing to construct ethnic identities amid a dominant white culture. The primary means of cultural expression during the Harlem Renaissance were literature and poetry, alth ough visual art, dramaRead MoreThe Harlem Renaissance941 Words à |à 4 PagesThe Harlem Renaissance was a cultural movement, in the early 1920ââ¬â¢s, that involved vibrancies of new life, ideas, and perceptions. The large migration of African Americans northward, after World War I, allowed people of color the opportunity to collaborate in the New York City neighborhood, known as Harlem. This renaissance allowed the city to thrive on a refined understanding and appreciation of the arts. Many individuals were involved in this movement including doctors, students, shopkeepers,Read MoreThe Harlem Renaissance1317 Words à |à 6 Pagesday is the Harlem Renaissance. The Harlem Renaissance is the cultural movement of the 1920ââ¬â¢s. The movement essentially kindled a new black cultural identity through art, literature and intellect. The Harlem Renaissance started during the Roaring Twenties. It took place in Harlem, New York. It became most prominent in the mid to late 1920ââ¬â¢s and it diminished toward the early 1930ââ¬â¢s (Henderson). The Harlem Renaissance was initially called the New Negro Movement or the New Negro Renaissance. It was theRead MoreThe Harlem Renaissance1586 Words à |à 7 PagesTHE HARLEM RENAISSANCE: ITS HISTORICAL IMPORTANCE ON BLACK CULTURE AND SOCIETY IN AMERICA Written by * Dr. William Mulligan History 522 Read MoreHarlem Renaissance3262 Words à |à 14 PagesHarlem Renaissance, a blossoming (c. 1918ââ¬â37) of African American culture, particularly in the creative arts, and the most influential movement in African American literary history. Embracing literary, musical, theatrical, and visual arts, participants sought to reconceptualize ââ¬Å"the Negroâ⬠apart from the white stereotypes that had influenced black peoplesââ¬â¢ relationship to their heritage and to each other. They also sought to break free of Victorian moral values and bourgeois shame about aspects ofRead MoreHarlem Renaissance1248 Words à |à 5 PagesHarlem Renaissance Brian Williamson Professor 11/25/2012 Strayer University Claude McKay was Jamaican American who moved from Jamaica to the United States in 1912. He attended the Tuskegee Institute in Alabama. This is where he received his first taste of racism here in America and this would have a drastic effect on his future writing. He left the Tuskegee Institute to attend school in Manhattan, Kansas. Mr. McKay then moved to New York invested in a restaurant and got married. The restaurantRead More The Harlem Renaissance Essay1145 Words à |à 5 PagesThe Harlem Renaissance In Harlem between the 1920ââ¬â¢s and 1930ââ¬â¢s the African American culture flourished, especially in areas such as music, art, literature, dance, and even in film. This soon became known as the Harlem Renaissance. With the entire positive and the negative situations of this time period the African Americans still seemed to have it all. The Harlem Renaissance came about because of the changes that had taken place in the African American community after the abolition of slavery
Tuesday, May 5, 2020
John Steinbecks Style Essay Sample free essay sample
Steinbeck is recognized as one of the Americaââ¬â¢s best short-story authors. Steinbeck wrote 32 books throughout his life. He is so celebrated and well-known they made a Museum after him. The National Steinbeck Center. He besides won many awards from his plants. Including. the Commonwealth of California Gold Medal for Tortilla Flats in 1935. This novel was his first widely known novel. In 1938 the book Of Mice and Men. was awarded the New York Drama Criticsââ¬â¢ Circle Award for the narrative of two farm workers seeking land of their ain. Besides. Steinbeckââ¬â¢s novels can be classified as societal novels covering with economic jobs. Such as his book Grapes of Wrath. this book earned him the Pulitzer Prize for fiction in 1940. Steinbeck is noted for his crisp. forceful parlance. dry wit. and profound compassion for the hapless. the inarticulate. and the politically oppressed. Another award that he earned was The Nobel Prize for Literature in 1962. Overall. Steinbeck has great novels and keeps the reader interested with the strong secret plans and complex characters. One book that I read from John Steinbeck that was merely late was Of Mice and Men. The narrative was these two cats seeking to win the American Dream. Their dream was to ain land/farm. This shows symbolism in that the dream farm of George and Lennie could stand for the American Dream and at the clip this dream was disillusioned with the thought of the Great Depression and how many could non in fact achieve their dream. The reader knew that something would forestall the two work forces from obtaining their dream. Symbolism is a Literary Device that John uses in most of his books. Besides to happen Steinbeck in his book is how close the scene is from his existent house. The bookââ¬â¢s scene was South of Soledad. California. Soledad is 30 stat mis off from Steinbeckââ¬â¢s place topographic point. Salinas. California. Steinbeck besides uses boding throughout the narrative. An illustration is. when Lennie reveals he has a dead mouse in his pocket. George inquiries him. ââ¬Å"Uh-uh. Jusââ¬â¢ a dead mouse. George. I didnââ¬â¢t kill it. Honest! I found it. I found it deadâ⬠( 5 ) this foreshadows the decease of Lennieââ¬â¢s puppy and the decease of Curleyââ¬â¢s married woman. ââ¬Å"The best-laid strategies of mice and work forces go oft awryâ⬠By Robert Burns means that even the best programs of mice and work forces could travel incorrect really easy. This means that George and Lennieââ¬â¢s dream didnââ¬â¢t go as planned. Another book made by John Steinbeck was The Red Pony. The scene is a spread in the California mountains ; Salinas. California. The subject is Jodyââ¬â¢s coming-of-age. and besides his altering relationship with his male parent. love. and loss of artlessness. Jody receives a gift by his male parent that symbolizes love and duty. Jody named the Equus caballus Gabilan. for Steinbeck lived near the Gabilan Mountains so that helped him pick out the name of the Equus caballus. This book shows boding excessively. ââ¬Å"Jody looked along the farm edifices. He felt an uncertainness in the air. a feeling of alteration and of loss and of the addition of new and unfamiliar thingsâ⬠( Steinbeck 4 ) . This foreshadows the decease of Gabilan and subsequently ( in ââ¬Å"The Promiseâ⬠) the birth of a new colt. Another literary device used is Simile. ââ¬Å"He [ Jody ] was merely a small male child. ten old ages old. with hair like dust-covered xanthous grassâ⬠¦Ã¢â¬ ( Steinbeck 2 ) . In the narrative Gabilan dies by catching a bad cold and the Equus caballus escapes the farm one dark and dies. John Steinbeck had the thought to do him ill because of his female parent which was deceasing of effects of a shot. One of the greatest books of John Steinbeck is the Grapes of Wrath. He wrote the book during the Great Depression. The book tells the truth about what happens in the great depression. It shows how the tone is plaintive. and sympathetic. The black drouth of the 1930s forces husbandmans to migrate west to California and Steinbeck uses realistic things in this novel such as Tom Joadââ¬â¢s urge to react to hardship and catastrophe by concentrating on oneââ¬â¢s ain demands and the urge to put on the line oneââ¬â¢s safety by working for a common good. Tomââ¬â¢s urge is to do life better and make what it takes to do it better. Agriculturists from the West accused Steinbeck of beliing their attitude toward migrators. This bookââ¬â¢s usage of profanity and the obscene stoping was burned when it was foremost published. For illustration in a descriptive scene. a auto hits and destroys Joadsââ¬â¢ Canis familiaris ( symbolic of the agony that lies in front for the household ) . A adult male bashes in Casyââ¬â¢s caput. and Tom avenges Casy by viciously crushing the adult male. This all show some type of imagination because you picture whatââ¬â¢s occurrence in the book. John Steinbeck uses literary devices as simile. personification. and boding. ââ¬Å"In the forenoon the dust hung like fog. and the Sun was every bit ruddy as mature new bloodâ⬠( p6 ) this is an illustration of a simile. For personification. ââ¬Å"The fire leaped and threw shadows on the houseâ⬠( p68 ) The household rescued by the benevolent alien at the terminal of Chapter 9 foreshadows the ââ¬Å"rescuingâ⬠of the Ha rriet wilsons by the Joads in the following chapter. This book has so many realistic events that really happened. but itââ¬â¢s a fiction book.
Friday, April 10, 2020
Small Scale Textile Industry free essay sample
The growth pattern of the Indian textile industry in the last decade has been considerably more than the previous decades, primarily on account of liberalization of trade and economic policies initiated by the Government in the 1990s. In producer-driven value chains, large, usually transnational, manufacturers play the central roles in coordinating production networks. This is typical of capital- and technologyintensive industries such as automobiles, aircraft, computers, semiconductors and heavy machinery. Buyer-driven value chains are those in which large retailers, marketers and branded manufacturers play the pivotal roles in setting up decentralized production networks in a variety of exporting countries, typically located in developing countries. This pattern of trade-led industrialization has become common in labour-intensive, consumer-goods industries such as garments, footwear, toys, handicrafts and consumer electronics. Large manufacturers control the producer-driven value chains at the point of production, while marketers and merchandisers exercise the main leverage in buyer-driven value chains at the design and retail stages. We will write a custom essay sample on Small Scale Textile Industry or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Apparel is an ideal industry for examining the dynamics of buyer-driven value chains. The relative ease of setting up clothing companies, coupled with the prevalence of developed-country protectionism in this sector, has led to an unparalleled diversity of garment exporters in the third world. Apparel is an ideal industry for examining the dynamics of buyer-driven value chains. Indiaââ¬â¢s textile industry comprises mostly smallscale, non-integrated spinning, weaving, finishing, and apparel-making enterprises. In this term paper, we study about the small scale Indian Textile Industry, its importance, role, roducts, finance, subsidies, attractiveness and the growth. The Textile industry has the potential to scale new height in the globalized economy. The textile industry in India has gone through significant charges in anticipation of increased international competition. The industry is facing numerous problems and among them the most important once are those of liquidity for many organized sector units, demand recession and insufficient price realization. The long-range problems include the need for sufficient modernisation and restructuring of the entire industry to cater more effectively to the demands of the domestic and foreign markets for textiles as per the needs of today and tomorrow.
Monday, March 9, 2020
Free Essays on Deborah Oropallo
ââ¬Å"WELCOME.â⬠In this painting, Oropalloââ¬â¢s used many implied lines to create the containers. We can actually see where the orange is touchin... Free Essays on Deborah Oropallo Free Essays on Deborah Oropallo Deborah Oropallo was born in Hackensack, New Jersey. According to the San Jose Museum of Art, Oropallo ââ¬Å"received her B.F.A. from Alfred University in New York State, and her M.A. and M.F.A. from the University of California, Berkeley.â⬠She now reside in Berkeley, California where she spends her days painting mundane object which slowly creating a striking image of poetic importance. She uses daily object such as ropes, tires, and doormats and transformed them into paintings where viewers can interpret a different meaning. In her most recent paintings, Oropalloââ¬â¢s uses the technique of digital photography and Iris prints. According to the LGI Digital & Fine Art Printing, ââ¬Å"Iris Printing offer richness, detail and depth that clearly set them apart from traditional offset lithography.â⬠Her paintings may seem far from sensuous paintings of other artist, yet the vibrant colors and suggestive overlays provide these objects visually dominant in a special way. Wh en she discovered this new way of art, she continue to discover the hidden beauty that lay in the objects that surround us in our daily life. She uses objects that are from our industrial surroundings to provide us with messages that she sees within our economy. She used object such as railroad tracks, flour packages, stacks of cinder blocks, buckets, pipes, and other large industrial containers that we customarily ignore, to enhance our knowledge of what is going on in our economy. In a painting by Oropallo entitled ââ¬Å"Oversize, 2001â⬠made with Iris Print from oil on a larger than life canvas, she utilized large containers in our industry and paints to come up with a message to everybody. We can see at the far left hand corner, the container is labeled as hazardous materials, but in front of the whole paintings, she places the words ââ¬Å"WELCOME.â⬠In this painting, Oropalloââ¬â¢s used many implied lines to create the containers. We can actually see where the orange is touchin...
Saturday, February 22, 2020
Racial Identity and Racial Identifications Essay
Racial Identity and Racial Identifications - Essay Example The two classification methods often cause confusion when it comes to racial identity. Some people may identify themselves with a given race based on its cultures. However, this identification may be very different from the color of their skin. In fact, they may call themselves black but phenotypically they are white in color. Misunderstandings and confusions on whether to identify oneââ¬â¢s race using external or internal characteristics have led to the aspect of racial identity being simplified and distorted by some people (Maureen) Simplification, in this case, is whereby an individual tries to define racial identity based on either external or internal characteristics alone. However, the definition fails to describe racial identity as a complex aspect of human nature may be affected by other factors. Many people would simply define racial identity as to refer to skin color while other would prefer to follow cultures. Others would even be distorted by the racial identity thing. ââ¬ËDistortedââ¬â¢ in this case meaning these people are mixed up and misunderstand the racial identity issue. ... This is a small boy who is trying to identify himself with a certain race. The boy is black in color but the fact that he described himself as black and white highlights the fact that the boy uses both internal and external characteristics of his racial identity. According to the author, the boy has both African and Irish roots. The author tries to convince the boy that he is black because of his skin color, but the boy insists that he is black and white. This shows how the writer simplifies the racial identity issue. Distortion comes in from the point of view of two different people that have different ways of defining racial identity. We are, therefore, not in a position to decide which of the two methods is best. Senna Describes herself as black in color but with parents from different races. The author writes of having a wasp mother and a black-Mexican father. She also describes herself as a black woman thereby introducing the use of external phenotypic characteristics to describ e racial identity. Her parents decided to raise her and her other siblings as black. This was not based on their skin color but their own choice. It was some sought of culture that believed that being black was a privilege and that black was beautiful too (Senna). In this case, there is the use of both external and internal characteristics for racial identity. These parents decided to raise their kids as black is a way of teaching the kids that black can be a culture. Therefore, the kids can racially identify themselves as black not only by the color of their skins but also by the type of culture they decide to embrace. This can be a simplified way of racial identification. However, it can be very hard to adopt for extremists who use color for racial identification.Ã
Thursday, February 6, 2020
The Marijuana Controversy Essay Example | Topics and Well Written Essays - 500 words
The Marijuana Controversy - Essay Example Another influencing factor in this regard is increased risk of accidents and criminal activity associated with marijuana. The proponents of legalization of marijuana, mainly the left wing activists and the Libertarians argue that the war pertaining to the drug is a failing one because; those imprisoned in regard to the drug are not cured of their addiction despite being in jail. Hence it would be more appropriate to legalize marijuana and control its transactions pertaining to purity, taxing and trafficking, like alcohol and tobacco. Legalization allows more control of the drug trafficking and safer and also more responsible use of the substance. The advocates of legalization also opined that crimes associated with marijuana would decrease in case it was legalized (Rawson, n.p). In about 14 states, mainly in the West, marijuana is allowed for medical purposes and several thousands of patients are seeking relief from many ailments through marijuana (Edmund, 5).
Tuesday, January 28, 2020
Challenging Characteristics of Online Learning Essay Example for Free
Challenging Characteristics of Online Learning Essay In online learning there are some challenging characteristics that an online learner need to face during their study periods among them are lack of motivation, stress, bad time management and learning style, computer illiterate, and language barrier. Firstly in lack of motivation, having lack of motivation is one of the characteristic challenges in online learning. If a student has lack of motivation this will make them less interested in their studies and this will drag the online learners down from producing good results in their studies. This is because without any motivation it will affect on how well the online learners do in their work and on when they will want to do their work and this also affects on how long will it take for them to work on the task given and this will automatically make the online learners lose their concentration on their studies. Next is when the online students are having stress during their study periods. Stress is an emotional challenge that the online learner faced in online learning. When one is having stress during his or her study period it is never a good sign because stress can affect both the body and mind. Warnings of stress such as significant changes in the behavior and life patterns can indicate that a person might be having stress. Among the symptoms of stress are the sudden bursts of anger, restlessness and or uneasiness, lack of joy, spontaneity, enthusiasm and or happiness. By having stress the online learner will have difficulty in concentrating on their studies as well as having trouble or problems in making decisions. Then there is time management and learning style. If the students or online learners are not well discipline and cannot manage time in a reasonable manner, he or she will not be able to cope up with their studies, work and personal life. A disorganized person is not going to have a good experience in their online learning. As an online student one need to have a good time management skills. An older student tends to manage their time properly because they have developed better time management skills. Therefore the younger generation of students mostly will have problems in managing their time accordingly. With a bad time management this will influence on the online learner learning skills. This is because with a bad time management the online learner will not be able to cope up with their studies and thus will make them lose their concentration in understanding their learning style. Meanwhile being a computer illiterate is also one of the challenges that people have with online learning. This is because not many people out there who are an online learner are computer literate. Most of the online learners are adults who might not be familiar with the recent technologies and software. Even though there are many computer literate students but not all of them possesses all the necessary skills needed in online learning. Some may still not know on how to use the Microsoft Office Words, Excels, and Power Point features and some online learners might have lack of technical problem solving skills and basic technology literacy skills such as file management. Lastly is the language barrier among the students and their study materials. This is also one of the challenges faced by the online learners in online learning especially in a non ââ¬â English speaking country like Malaysia. Most of the references retrieved by the online learners online are in English and some of the teaching materials provided for the online learner are also in English. Growing up with a non ââ¬â English native mother tongue will somehow give trouble for some students to understand their study materials. They will need to take more time than other students to understand their study materials. So these will one way or another lower their self esteem and their confidence in doing the task given to them.
Monday, January 20, 2020
The Mystery of What is Normal Essay -- Normality Family Essays
The Mystery of What is ââ¬Å"Normalâ⬠In order to think about whether someoneââ¬â¢s family is ââ¬Å"normalâ⬠or not, you would have to consider many factors.ââ¬Å"Normalâ⬠in what sense of the word?What aspect of the family are we considering the normality?Are we talking about the familyââ¬â¢s culture, quality of living, habits, the way that the present themselves, or are we just comparing them to the people next door?Are we talking about the normality of the family at face value or are we asking about the normality of that family which only members of that family have experienced?There are so many definitions of the word ââ¬Å"normal.â⬠Finding a definition of the word ââ¬Å"normalâ⬠depends on the personââ¬â¢s definition of what he or she thinks ââ¬Å"normalâ⬠means. In the dictionary, it states that the definition of ââ¬Å"normalâ⬠is ââ¬Å"Conforming with, adhering to, or constituting a norm, standard, pattern, level, or type; typical.â⬠But the word itself has a wide range of meanings.It can mean: ââ¬Å"whatââ¬â¢s accepted,â⬠ââ¬Å"average,â⬠ââ¬Å"just like everyone else,â⬠or ââ¬Å"just not sticking out in the crowdâ⬠just to name a few. We all have different perceptions of what the word normal means, and what is considered to be different.This perception is always changing and is affected by everything around us.If you ask a person what is normal one day, and then ask him again in about a month, that person will probably give an entirely different answer.The word normal is, in the most part, has opinionated definition.It varies from person to person, and changes dramatically as each person learns, experiences and accepts new things. Now that that has all been said, how can I consider if my family is normal or not?If the definition of the word normal varies from person to person, my answer to the ... ...ââ¬Å"devil cat?â⬠How many people have a father with an explosive temper?How many people have a mother who is a supervisor in a party plan?Iââ¬â¢m sure that every person has a family that has differences that ranges from beliefs to habits to any experience of even the smallest significance. The strongest word that I would use to compare anyoneââ¬â¢s family is the word ââ¬Å"similar.â⬠No family is ââ¬Å"normal.â⬠I myself have a hard time using the word ââ¬Å"normal.â⬠The only way that a family could be considered as normal is if every family was exactly alike, and alike in everyway possible.There is no true definition of the word ââ¬Å"normalâ⬠in a society where whatââ¬â¢s frowned upon one day is commonplace the next, and vice versa. Works Cited Bass, Randal. ââ¬Å"Borders as Barriers: Otherness and Difference.â⬠Bordrtext: Cultural Reading for Contemporary Writers. Boston: Houghton Mifflin, 1999. 205-210
Sunday, January 12, 2020
Morals and ethics essay
The topics that are going to be explained include- the abortion policy, Bursas and how society deals with rapes. It will summarized Into a short paragraph for each topic which will Include an extra paragraph for defining laws and how they fit Into society, also a paragraph to explain what morals and ethics and how they have changed through society. The law Is a dynamic thing. Let a complex mechanism evolving from hundreds of years of tradition, culture and values.In general terms, the law can be defined as a set of enforceable rules of conduct with set down guidelines for relationships between people and organisms in a society. Laws change when society does, when a certain law is changed to fit the modern day society, it is a very complicated process in which a law needs to be changed. For example, Australian politician Barry Farrell has changed the law of selling drinks in bars after a certain time. The law states that bars and puns are not allowed to sell drinks after am to reduce people from being injured or even killed.Ethics and morals both relate to ââ¬Å"rightâ⬠and ââ¬Å"wrongâ⬠conduct. Ethics Is set by a series of rules provided to an individual by an external source, egg- their profession. Whereas, morals refer to as an Individual's own principles regarding right and wrong. Ethics do not change as a person moves from one society to the next. Morals may change as a person moves from one society to the next. Bursas are an important point towards changing the community over time. The Burk is a fully covered outer garment that Islamic traditions wear to cover up their dies, showing only their eyes.The problem regarding the Bursas is that this tradition has been brought into Australia and worn by Muslim women everywhere, and refuse to take it off. This is why a new law is trying to be enforced to make sure Muslim women take off their Bursas when asked or needed too. The legislation was drafted in response to public outcry about the case of Bur k-weaning mother-of- seven Carnet Matthews, who had a conviction of knowingly making a false statement quashed.Ms Matthews was originally given a six-month Call sentence after being mound guilty of falsely accusing a senior constable of forcibly trying to remove her Burk when she was pulled over while driving in Woodbine in Kidney's southwest in June 2010. She was later found innocent on appeal after the prosecution could not prove she was the woman who signed the statement while wearing the garment. This statement is the main reason why Bursas should be taken off when told of. Abortion is also another significant topic in relate to change in the community.The grounds on which abortion is permitted in Australia vary from state to state. In every Tate, abortion is legal to protect the life and health of the woman, though each state has a different definition. There is now law anywhere in Australia that requires the notification or consent of a woman's sexual partner. There Is also no enforced waiting period or an abortion, except In Western Australia, a minor does not require parental consent or notification. This law Is also very similar to the one about rape. They both are salary In some ways and could not be controlled at sometimes.This law has changed over the last 40 years as back in the sass's, abortions were illegal in omen being bashed and then raped and getting pregnant, they had to change the law because of this. A woman will have to have the pregnancy and live her whole life knowing how her child was brought into the world. Every time she looks at her child it could remind her of that horrible and unforgettable night. Another reason this law had been dropped was because of several gang rapes around Sydney. There was a number of attacks in a matter of weeks involving a group of guys on a girl in 2000.Eight Lebanese men kept approaching two young girls aged 17 and 18 in a car on August 10th, August 12th, a 16 year old girl was walking through a park wh en a 17 year old raped her alongside 12 other men and one had even held a gun against her head, August 30th, a woman was told she was being taken to a house to smoke some cannabis, however she was taken to three different houses where she was raped 25 times by 14 different men, two 16 year old girls were taken by attackers at a local train station, where then three older men raped then repeatedly over five hours. This just goes to show how easy it could be to get pregnant after being raped.This tenement is true, this was happening to people because of the policy. In Conclusion, given the evidence, moral and ethics have changed law in modern society for a number of different reasons. The law regarding the Burk is still being questioned and still has many different ways n how to change or stop the law. Abortion has changed due to young women being raped, because of this the government and communities want this horrible thing to stop. Also, people are generally pleased with the law reg arding abortion as people do not want to see young teens being forced to have and live with a child.
Saturday, January 4, 2020
DNA Structure Chains - Free Essay Example
Sample details Pages: 6 Words: 1829 Downloads: 10 Date added: 2017/06/26 Category Biology Essay Type Research paper Tags: DNA Essay Did you like this example? 1. Introduction 1.1 DNA Structure DNA is a polymer made of subunits called as nucleotides. Each nucleotide consists of a deoxiribose sugar, a phosphste, and a nitrogenous base (Genetics from Genes to Genomes). Donââ¬â¢t waste time! Our writers will create an original "DNA Structure Chains" essay for you Create order Watson and Crick proposed the structure for DNA (shown schematically in Figure 1 a). This is the presence of two polynucleotide strands coiling around a common axis and those strands linked together by a specific hydrogen bond scheme between the purine and pyrimidine bases (Figure 1 b), viz. adenine (A) with thymine (T) and guanine (G) with cytosine (C). The carbon atoms of the deoxyribose sugar are distinguished from atoms of the deoxyribose within the nucleotide base by the use of primed numbers from 1-5. The phosphodieser bonds always form a covalent link between the 3 carbon of one nucleoside and the 5 carbon of the following nucleoside. The consistent orientation of the nucleotide building blocks gives a chain overall direction, such that the two ends of a single chain are chemically distinct. At the 5 end, the sugar of the terminal nucleotide has a free 5 carbon atom and at the other 3 end of the chain, it is the 3 carbon of the final nucleotide that is free (Genetics from Genes to Genomes). In the model, two DNA chains spiral around an axis with the sugar-phosphate backbones on the outside and pairs of bases (one from each chain) meeting in the middle. Although both chains wind around the helix axis in a right-handed sense, chemically one of them runs 5 to 3 upward, while the other runs in the opposite direction of 5 to 3 downward. In short, the two chains are antiparallel. The base pairs are essentially flat and perpendicular to the helix axis, and the planes of the sugars are roughly perpendicular to the base pairs. As the two chains spiral about the helix axis, they wrap around each other spiral about the helix axis, they wrap around each other once every 10 base pairs, or once every 34ÃÆ'â⬠¦ (Genes to genomes). In a space-filling representation of the model, the overall shape is that of a cylinder with a diameter of 20ÃÆ'â⬠¦ whose axis is the axis of the double helix. The backbones spiral around the axis like threads on a screw, but because there are two backbones, there are two threads, and these two threads are vertically displaced from each other. This displacement of the backbones generates two grooves, one much wider than the other, that also spiral around the helix axis. Biochemists refer to the wider groove as the major groove and the narrower one as the minor groove. The two chains of double helix are held together by hydrogen bonds between complementary base pairs, A-T and G-C. Since the overall shapes of the two base pairs are quite similar, either pair can fit into the structure at each position along with DNA. Moreover, each base pair can be accommodated in the structure in two ways that are the reverse of each other: an A purine may be on strand 1 with its corresponding T pyrimidine on strand 2 or the T pyrimidine may be on strand 1 and the A purine on strand 2. In addition A-C and G-T pairs do not fit well together; that is, they do not easily form hydrogen bonds. The DNA molec ule is essentially a polynucleotide or a polymer chain formed by phosphate diester groups joining b-D-deoxyribose sugars through their 3 and 5 hydroxyl groups (Figure 3). The backbone of the DNA molecule thus consists of six single bonds about which rotations can take place (also indicated in Figure 3) giving rise to various possible conformations/structures for the polymeric chain. As mentioned above, the canonical Watson-Crick DNA model is a two-stranded helical structure, in which the two chains are held together by hydrogen bonds between the purine (A,G) and pyrimidine (T,C) bases. There are 10 nucleotides per turn, separated by + 36 rotation and 3.4 ÃÆ'â⬠¦ translation along the helix axis,in each of the two chains and the two chains are aligned in mutually anti-parallel orientations (Figures 1 a and 4) (Manju Bensal) DNA can inter-convert between two well-defined forms, viz. A and B (Figure 2). The molecular structures corresponding to these two forms were later show n to be essentially similar in their handedness, chain orientation and hydrogen bonding scheme. Subsequently it has become clear that the DNA molecule has an enormous ability to undergo structural changes depending on its environment by twisting, turning and stretching, leading to a pantheon of DNA structures6. Several of these structural polymorphs of DNA have now been experimentally characterized using X-ray diffraction, NMR or other spectroscopic studies and are found to vary considerably from the Watson-Crick type structure (Manju Bensal). 1.2 Principle of Agarose Gel Electrophoresis Electrophoresis is defined as movement of small ions and charged molecules in solution under the influence of an electric field (Gel Electrophoresis of Nucleic Acid, A Practical Approach). Agarose gel electrophoresis is a widely used method that separates molecules based upon charge, size and shape. It is particularly useful in separating charged biomolecules such as DNA, RNA and proteins (Lab Electrophoresis). Agarose gel electrophoresis possesses great resolving power, yet is relatively simple and straightforward to perform. The gel is made by dissolving agarose powder in boiling buffer solution. The solution is then cooled to approximately 55oC and poured into a mol containing a comb that makes well when solution is polymerised (Lab Electrophoresis). Electrophoresis is carried out in the gels cast either in tubes or as slabs. A number of gel materials have been used successfully, including agar, agarose, and polyacrylamide. Agar and agarose gels are made by heating the gra nular material in the appropriate electrolyte buffer, casting the gels and allowing them to set on cooling. The resolving power of these gels depends on the concentration of dissolved agar or agarose; dilute gels are used for very large DNA molecules and more concentrated gels for low molecular weight DNA (Gel Electrophoresis of Nuclecic Acid, A Practical Approach). Samples are prepared for electrophoresis by mixing them with components that will give the mixture density, such as glycerol or sucrose. This makes the samples denser than the electrophoresis buffer. These samples can then be loaded with a micropipette or transfer pipet into wells that were created in the gel by a template during casting. The dense samples sink through the buffer and remain in the wells (Lab Electrophoresis). A direct current power supply is connected to the electrophoresis apparatus and current is applied. Charge molecules in the sample enter the gel through the walls of the wells. Molecules ha ving a net negative charge migrate towards the positive electrode (anode) while net positively charged molecules migrate towards the negative electrode (cathode). Within a range, the higher the applied voltage, the faster the samples migrate. The buffer serves as a conductor of electricity and to control pH. The pH is important to the charge and stability of biological molecules (Lab Electrophoresis). The rate of migration depends on the size and shape of the molecule, the charge carried. In an electric field at moderate pH, negatively-charged DNA molecules migrate towards the anode. A fractionation is achieved because large molecules move more slowly through the gel than small molecules and selection of DNA within a given size range is obtained by selecting a gel of appropriate pore size. Electrolytes used in electrophoresis generally consist of an aqueous buffer, containing a chelating agent such as ethylenediaminetetractate (EDTA) and a nuclease inhibitor such as sodium dodecyl sulphate (SDS). A number of factors affect the fractionation of RNA. Increasing the current leads to higher rates of migration, but the flow of current also results in the production of heat, which, if excessive, adversely affects the separation by causing trailing and broadening of the zones. 1.3 Fluorescence 1.3.1 Silver Staining Ethidium bromide staining is the conventional laboratory technique for the detection of DNA. Switzer et al (1979) originally introduced silver staining technique for the detection and analysis of proteins. Currently, silver staining is sometimes used to detect DNA fragments, including short interspersed nuclear elements, VNTR detection, and SNPs in various experiments (Yan-Chung Han et al, 2008). The new method is much efficient and sensitive for polymorphism DNA analysis and the detection of small amount of nucleic acid (Sommerville and Wang, 1981; Boulikas and Hancock, 1981; Goldman and Merril, 1982; and Guillemette and Lewis, 1983) but more versatile silver staining is needed for analysis of complex DNA profiles generated in DNA amplification fingerprinting and DNA sequencing (Anolles and Gresshoff, 1994). The ethidium bromide staining of DNA is time-consuming as they require a lot of preparation and handling of several solutions prior to use and needs expensive and bulky fluorescence imaging equipment. Furthermore, the sensitivity, color uniformity, and storage time of the staining gels are not ideal (Yan-Chuang Han et al, 2008). Moreover, Ethidium bromide used for staining of DNA as a conventional method is a carcinogenic substance and a cost of waster disposal. Many modifications to silver staining method have been reported since the introduction of silver staining method for the analysis of proteins and nucleic acid analysis in agarose and polyacrylamide gels. It has been reported that some procedures used to stain nucleic acids in polyacrylamide gels are not suitable for agarose gels due to differences in the chemical compositions of both matrices. The agarose matrix has the disadvantage of nonspecific depositions of silver ions resulting in high background (Willoughby and Lambert 1983, Peats 1983, and Lasne et al 1983). Those protocols developed in order to reduce the nonspecific stain in agarose gels involve time-consuming pretreatment s teps with K2Cr2O7 or Na2S2O3 (Zalazar et al, 2001). During image development almost all staining procedures reduce silver ion to colloidal silver, which is then deposited in the immediate vicinity of the staining substratum. For optimal image contrast, the level of silver reduction in the gel matrix must be kept to a minimum level. This is performed by appropriate modulation of the speed of the reduction process, which depends mainly on the pH, the absolute and relative concentrations of silver and reducing agents, and the rate constant of the reaction (Anolles and Gresshoff, 1994). Silver staining is also useful for the microarray technology in order to impede the interference of fluorescent label with the hybridisation process. This advantage is achieved by application of silver staining after the hybridization process. Silver staining also eliminates the need of fluorescence imaging equipment in microarray technology by means of using a film scanner. The aims of the pres ent study are to optimize a silver staining protocol performed for a commercially obtained DNA molecular weight marker, in which the procedure is modified. The detection limit of silver staining is investigated and is compared with ethidium bromide staining. Moreover, some of the siver staining methods are varied and are compared with ethidium bromide staining. The silver staining protocol is modified inorder to increase the sensitivity and reduce background staining. After a suitable protocol is optimized DNA are deposited on filter paper at various dilutions and stained with the optimized. This has implications for the development of portable biosensors with label free detection.
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